RDGAWKY gene targeted medicament for preventing and treating grain storage pest beetle and application thereof

The invention provides an RDGAWKY gene targeting agent for preventing and controlling grain storage insect grain beetles and application of the RDGAWKY gene targeting agent, and belongs to the technical field of grain storage insect prevention and control. The RDGAWKY gene targeting agent for preven...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: XU HONGLEI, TANG PEI'AN, WU XUEYOU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides an RDGAWKY gene targeting agent for preventing and controlling grain storage insect grain beetles and application of the RDGAWKY gene targeting agent, and belongs to the technical field of grain storage insect prevention and control. The RDGAWKY gene targeting agent for preventing and treating grain storage pest beetle is prepared by adopting a method comprising the following steps: carrying out reverse transcription on total RNA (Ribonucleic Acid) of the beetle into cDNA (Complementary Deoxyribose Nucleic Acid), carrying out PCR (Polymerase Chain Reaction) amplification by taking the cDNA as a template and adopting a primer pair as shown in SEQ ID No.1 and SEQ ID No.2, and carrying out in-vitro transcription on a PCR amplification product to synthesize dsRNA, so as to obtain the RDGAWKY gene targeting agent. After the RDGAWKY gene targeted medicament for preventing and treating the grain storage pest beetles is applied, the grain storage pest beetles can be effectively prevented and tr