Culture method of pathogenic tissue poison

The invention discloses a culture method of a pathogenic tissue virus. The culture method comprises the following steps: S10, obtaining a seed virus of porcine respiratory diseases; s20, injecting the obtained seed virus into the body of the pig from the trachea of the pig to obtain an experimental pig with the corresponding disease symptom; and S30, when the experimental pig is fed until the disease symptom of the experimental pig begins to relieve, dissecting the experimental pig to obtain the pathogenic tissue poison. Trachea inoculation is carried out in the sober state of the experimental pig, the suffocation condition caused by the fact that seed venom intensively enters lungs during anesthesia can be avoided, meanwhile, trachea inoculation is adopted, target tissue can be directly reached, loss is small, and the seed venom demand is low; by diluting the seed virus, the volume is increased, the seed virus can flow into more bronchus and contact more target cells, and the preparation efficiency is improv