Method for inducing neural stem cell spheres into neurons
The invention discloses a method for inducing neural stem cell spheres into neurons, which comprises the following steps: taking a single-cell suspension of the neural stem cell spheres, resuspending by using a neuron culture medium, inoculating to a pre-coated cell culture plate, and carrying out i...
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| creator | ZHU ZIWEI GUO HONGYU CHEN XIAOWEI YANG MIN ZHANG ZIRUI ZHANG HUANHONG LIU HAORAN |
| description | The invention discloses a method for inducing neural stem cell spheres into neurons, which comprises the following steps: taking a single-cell suspension of the neural stem cell spheres, resuspending by using a neuron culture medium, inoculating to a pre-coated cell culture plate, and carrying out induced culture for 10 days under the conditions of 37 DEG C and 5% CO2 to obtain the neurons. The single-cell suspension of the neural stem cell spheres is obtained by the following method: collecting human suspension cultured cell spheres, centrifuging, discarding the supernatant, adding Accutase enzyme, oscillating and incubating for 10-30 minutes at the constant temperature of 37 DEG C, and blowing and beating into the single-cell suspension by using a pipette. According to the method, the neurons are directly induced by the human neural stem cell spheres, the neurons do not need to be obtained through a transfection technology, and the conditions that other cells are induced into neural stem cells and then indu |
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The single-cell suspension of the neural stem cell spheres is obtained by the following method: collecting human suspension cultured cell spheres, centrifuging, discarding the supernatant, adding Accutase enzyme, oscillating and incubating for 10-30 minutes at the constant temperature of 37 DEG C, and blowing and beating into the single-cell suspension by using a pipette. 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The single-cell suspension of the neural stem cell spheres is obtained by the following method: collecting human suspension cultured cell spheres, centrifuging, discarding the supernatant, adding Accutase enzyme, oscillating and incubating for 10-30 minutes at the constant temperature of 37 DEG C, and blowing and beating into the single-cell suspension by using a pipette. According to the method, the neurons are directly induced by the human neural stem cell spheres, the neurons do not need to be obtained through a transfection technology, and the conditions that other cells are induced into neural stem cells and then indu</abstract><oa>free_for_read</oa></addata></record> |
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| title | Method for inducing neural stem cell spheres into neurons |
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