Method for inducing neural stem cell spheres into neurons

The invention discloses a method for inducing neural stem cell spheres into neurons, which comprises the following steps: taking a single-cell suspension of the neural stem cell spheres, resuspending by using a neuron culture medium, inoculating to a pre-coated cell culture plate, and carrying out induced culture for 10 days under the conditions of 37 DEG C and 5% CO2 to obtain the neurons. The single-cell suspension of the neural stem cell spheres is obtained by the following method: collecting human suspension cultured cell spheres, centrifuging, discarding the supernatant, adding Accutase enzyme, oscillating and incubating for 10-30 minutes at the constant temperature of 37 DEG C, and blowing and beating into the single-cell suspension by using a pipette. According to the method, the neurons are directly induced by the human neural stem cell spheres, the neurons do not need to be obtained through a transfection technology, and the conditions that other cells are induced into neural stem cells and then indu