Three-dimensional induction method for obtaining mesenchymal stem cells through differentiation of pluripotent stem cells

The invention discloses a three-dimensional induction method for obtaining mesenchymal stem cells through differentiation of pluripotent stem cells, and relates to the technical field of biomedical engineering.The three-dimensional induction method for obtaining the mesenchymal stem cells through differentiation of the pluripotent stem cells comprises the following steps that S1, iPSC cells are subjected to resuscitation and inoculation; s2, culturing the iPSC cells and changing the liquid; s3, carrying out induced differentiation on the iMSC; starting preparation software, automatically pumping an iMSC first-stage induction culture medium into the full-automatic preparation system, and carrying out continuous induction culture; after the iMSC induction culture is carried out for a period of time, the iMSC second-stage induction culture medium is completely replaced; s4, carrying out iMSC amplification; s5, cryopreserving the iMSC; a full-automatic cell preparation system is combined with a vertical wheel bio