In-vitro culture method of chicken pluripotent stem cells

The invention discloses a chicken pluripotent stem cell in-vitro culture method, and belongs to the technical field of animal husbandry and biology. FGF2, IWR-1 and XAV-93 are added, STO cells serve as a feeding layer, an FIX culture system is established, embryonic disc bright region cells are separated from fresh chicken hatching eggs in the EGK.X period, in the FIX culture system, culture is conducted in an incubator with 5% CO2 and saturated humidity and the temperature of 37 DEG C, a culture solution is replaced every day, passage is conducted once every 2-3 days, and in-vitro culture of the chicken pluripotent stem cells PSCs is conducted. According to the method provided by the invention, the chicken PSCs can be rapidly proliferated in a short time, and the pluripotency and differentiation potential of the chicken PSCs can be maintained; materials are provided for studying the biological characteristics of the chicken PSCs, a new method is provided for poultry improved variety propagation, gene modific