Composite liposome adjuvant, preparation method and application of composite liposome adjuvant in foot-and-mouth disease subunit vaccine

On one hand, the invention discloses a composite liposome adjuvant and a preparation method thereof. The encapsulation efficiency of the composite liposome adjuvant is 90.08%, the particle size is 110 +/-20 nm, and the potential is-20.30 +/-10.65 mV. The invention also discloses a foot-and-mouth disease subunit vaccine. The vaccine contains the composite liposome adjuvant and foot-and-mouth disease VLPs. The composite liposome adjuvant obtained by the test has good encapsulation efficiency and small nano particle size. And the preparation method of the composite liposome adjuvant is simple, easy to amplify and suitable for large-scale production and application. The foot-and-mouth disease subunit vaccine prepared by using the composite liposome adjuvant prepared by the invention is used for immunizing healthy pigs; and after immunization, the titer of the ELISA antibody and the titer of the neutralizing antibody of the experimental group are higher than those of the commodity group. Therefore, the prepared co