Method for verifying specificity of virus titer of Japanese encephalitis live attenuated vaccine

The invention discloses a method for verifying specificity of virus titer of Japanese encephalitis live attenuated vaccines, which comprises the following steps: 1) preparing a test group solution, 2) preparing a control group solution, 3) preparing a neutralization group solution, 4) respectively taking the test group solution obtained in the step 1), the control group solution obtained in the step 2) and the neutralization group solution obtained in the step 3), inoculating to a BHK21 cell plate growing into a single layer, and carrying out freeze drying to obtain the virus titer specificity of the Japanese encephalitis live attenuated vaccines. After adsorption, adding a nutrient covering for culture, dyeing, and observing BHK21 cells and plaques. When the method is applied to virus titration specificity verification of the Japanese encephalitis live vaccine, the quality of the Japanese encephalitis live vaccine can be effectively controlled, and the method has popularization and application values. 本发明公开了