Meloidogyne incognita primer probe combination and detection method based on microdroplet type digital PCR (Polymerase Chain Reaction) technology

The invention relates to the technical field of gene engineering, in particular to a meloidogyne incognita primer probe combination and a detection method based on a microdroplet type digital PCR (Polymerase Chain Reaction) technology. Comprising a primer pair and a probe, the nucleotide sequence of an upstream primer of the primer pair is as shown in SEQ ID No. 1; the nucleotide sequence of a downstream primer of the primer pair is as shown in SEQ ID No.2; the nucleotide sequence of the probe is as shown in SEQ ID No. 3. The microdroplet type digital PCR technology is applied to detect the meloidogyne incognita, and compared with a detection method of a RAPD technology and a ribosome DNA restriction enzyme technology which are poor in repeatability, the microdroplet type digital PCR technology can save time, is accurate and sensitive, and can establish a rapid and accurate meloidogyne incognita detection technology. The technology can be applied to early-stage rapid molecular detection of meloidogyne incogni