Application of anti-IgM antibody coupled agarose microspheres in purification of extracellular vesicles
The invention discloses an application of anti-IgM antibody coupled agarose microspheres in purification of extracellular vesicles. The anti-IgM microspheres can specifically bind and remove residual high-abundance IgM particle pollutants in an EV product, the total protein amount of the product can...
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| creator | ZHANG JIAYI GUO JIAHUI CUI YIZHI WANG TONG XIAO XUHUANG |
| description | The invention discloses an application of anti-IgM antibody coupled agarose microspheres in purification of extracellular vesicles. The anti-IgM microspheres can specifically bind and remove residual high-abundance IgM particle pollutants in an EV product, the total protein amount of the product can be reduced by 44.7%, no negative influence is generated on EV in the product, and the purity of the EV is effectively improved; the SEC column added with the anti-IgM microspheres synchronously realizes separation and purification of EV, free proteins including IgM in a body fluid sample are intercepted, and high-purity EV can be obtained through one-step elution. The method is a simple and convenient EV purification/separation technology, can be compatible with various conventional EV separation technologies when being used as a purification means, and effectively relieves pollution of IgM particles with extremely high abundance in samples such as serum to the samples; when used as a separation technology, the me |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Application of anti-IgM antibody coupled agarose microspheres in purification of extracellular vesicles |
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