Method for simultaneously producing high-titer Seneca valley virus and hog cholera virus antigens

The invention discloses a method for simultaneously producing high-titer Seneca valley virus and hog cholera virus antigens. The method comprises the following steps: preparing a suspended BHK-21 cell strain by using a bioreactor, respectively inoculating Seneca grain virus and hog cholera virus, setting the control parameters of the bioreactor after virus inoculation as follows: DO (dissolved oxygen) 30-60%, pH (potential of hydrogen) 7.0-7.4, stirring speed of 50-100rpm/min and temperature of 36.5-37.5 DEG C, sampling at regular time during the period, dyeing with 0.4% trypan blue to observe cytopathy, and harvesting a suspended culture virus solution when the cell viability is less than or equal to 20%. The method realizes large-scale breeding and culture of the hog cholera virus and the Seneca valley virus at the same time, has the advantages of simple and convenient process, low cost, stable toxin production effect, high titer and the like, and lays a foundation for large-scale production of a later-stag