Temperature compensation method and system for digital PCR (Polymerase Chain Reaction) of medical equipment and medium

The embodiment of the invention provides a medical equipment digital PCR temperature compensation method and system and a medium, and the method comprises the steps: collecting the current temperatures of different temperature points of a PCR instrument, carrying out the difference calculation of th...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: LYU XIAOJUN, HE LEI, ZHONG ZHIGANG, XU XIUYAO
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The embodiment of the invention provides a medical equipment digital PCR temperature compensation method and system and a medium, and the method comprises the steps: collecting the current temperatures of different temperature points of a PCR instrument, carrying out the difference calculation of the collected current temperatures of the temperature points and a target temperature, and obtaining the temperature difference information; generating a mirrored thermodynamic diagram by using a pixel clustering method, and performing point-to-point temperature compensation on different temperature points of the PCR instrument according to the thermodynamic diagram; obtaining a temperature difference extreme value of the current temperature and the target temperature of the same temperature point after temperature compensation; if the temperature difference extreme value is smaller than the set temperature difference threshold value, temperature compensation is stopped, the temperature abnormity of the temperature p