Kit for detecting inversion of intron 1 and intron 22 of F8 gene and application of kit

The invention relates to a kit for detecting inversion of an intron 1 and an intron 22 of an F8 gene and application of the kit. Specifically, the invention provides a primer pair set and a kit which are rapid, efficient and high in specificity and can be used for detecting inversion of the intron 1 and the intron 22 of the human F8 gene. The primer set provided by the invention can be used for efficiently and specifically amplifying, the detection band is clear, and no non-specific band exists. The kit provided by the invention has the advantages of simple components, simplicity in operation, rapidness in detection, capability of effectively reducing the cost and the like. 本发明涉及一种检测F8基因1号内含子和22号内含子倒位的试剂盒及其应用。具体地，本发明提供一种快速、高效、特异性强的可用于检测人F8基因1号内含子和22号内含子倒位的引物对集和试剂盒。本发明提供的引物集可高效、特异性的进行扩增，检测条带清晰，无非特异性条带。本发明提供的试剂盒具有组分简单、操作简单、检测快速以及能够有效降低成本等优点。