Single cell protein analysis method and system

Provided herein are methods and compositions for separating and analyzing cells. The separation method comprises encapsulating individual cells in droplets, enabling biomolecular analysis at the level of single cells. The concept is further that various biomarkers can be quantified from a single red...

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Bibliographische Detailangaben
Hauptverfasser: SWAMINATHAN JAGANNATH, BALDO ANDREA, SALEY WILLIAM, FLOYD, BRIAN, ANSLIN ERIC VINCENT, MARCOTTE EDWARD, HOWARD II CHARLES JOHN, TANG VINCENT QUOC TRUNG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:Provided herein are methods and compositions for separating and analyzing cells. The separation method comprises encapsulating individual cells in droplets, enabling biomolecular analysis at the level of single cells. The concept is further that various biomarkers can be quantified from a single red blood cell, including methods that can determine whether a subject has undergone autoblood transfer. 本文提供了用于分隔和分析细胞的方法和组合物。所述分隔方法包括将单个细胞包封在微滴中,使得能够在单细胞水平上进行生物分子分析。该概念进一步的是,可以从单个红血细胞量化多种生物标志物,包括可以确定受试者是否已经经历自体血液转移的方法。