Multiple Q-PCR detection kit for HPV virus and preparation thereof

The invention discloses a multiplex Q-PCR (Quantitative Polymerase Chain Reaction) detection kit for HPV (Human Papilloma Virus) and a preparation method thereof. The detection kit simultaneously and respectively comprises Q-PCR primer pairs of HPV16/18/11, HPV31/52/6 and HPV33/45/58, which do not interfere with each other and do not influence each other, so that HPV16, HPV18, HPV11, HPV31, HPV52, HPV6, HPV33, HPV48 and HPV58 can be simultaneously and accurately detected in one step; the kit comprises a PCR buffer solution, a freeze-drying protective agent, Taq DNA polymerase, UDG enzyme, a human papilloma virus typing probe, dNTP, a reconstitution fluid, a positive reference substance and a blank reference substance. The freeze-dried sample is prepared by mixing the components, sub-packaging the components into PCR tubes for bead making, and freeze-drying the beads in a freeze dryer, so that the dependence on a cold chain in the transportation and storage process is avoided, and the quality stability is guar