Method for rapidly extracting DNA suitable for high-throughput sequencing from Trizol sample

The invention belongs to the technical field of biology, and particularly relates to a method for rapidly extracting DNA suitable for high-throughput sequencing from a Trizol sample. The method specifically comprises the following steps: carrying out ethanol precipitation and rinsing on Trizol homogenate of an interlayer/organic layer containing DNA, and dissolving with NaOH to obtain a neutralized solution; adding protease K into the obtained solution, and performing water bath digestion to obtain a reaction solution; adding a DNA binding solution into the reaction solution, then adding isopropanol, uniformly mixing, adding a purification column, and centrifuging; adding DNA rinsing liquid into the purification column, and centrifuging; adding water into the purification column for elution, standing and centrifuging to obtain a DNA sample. A DNA crude product is digested by high-concentration protease K, and an efficient and convenient nucleic acid silica gel column recovery system compatible with the DNA cr