Santalol biosensor and application thereof

The invention discloses a santalol biosensor and application, and relates to the field of bioengineering. According to the invention, four promoters responding to santalol are screened for the first time, a red fluorescent protein expression box is constructed by the promoters, the positive correlat...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: LIAN JIACHANG, ZUO YIMENG
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a santalol biosensor and application, and relates to the field of bioengineering. According to the invention, four promoters responding to santalol are screened for the first time, a red fluorescent protein expression box is constructed by the promoters, the positive correlation between the santalol concentration and the red fluorescent protein intensity within a certain range is established, a santalol biosensor is established for the first time, and the technical barrier in the field is broken. The constructed santalol biosensor is a product response type biosensor and is rarely reported, the biosensor is used for screening santalol high-yield strains, and the technical problems that santalol is colorless, lack of chromogenic reaction and can only be quantified through mass spectrometry are solved. The structural formula of the santalol biosensor nucleic acid constructed by the invention is P-RP-T, and RP can be replaced by an antibiotic resistance gene, so that the positive correlat