Recombinant bacterium and method for producing BT toxin by using same

The invention provides a recombinant bacterium and a method for producing BT toxin by using the recombinant bacterium, the method comprises the following steps: introducing a sequence for coding BT toxin into halomonas, then culturing the halomonas in a culture medium and inducing BT toxin expressio...

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Bibliographische Detailangaben
Hauptverfasser:	CHEN GUOQIANG, LIU XU, GUO WEIKE
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERTILISERS FERTILISERS CHARACTERISED BY THEIR FORM FISHING FORESTRY HUMAN NECESSITIES HUNTING MANUFACTURE THEREOF METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENTSUBCLASSES OF CLASS C05 MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVINGA SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES,SOIL-CONDITIONERS, WETTING AGENTS MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a recombinant bacterium and a method for producing BT toxin by using the recombinant bacterium, the method comprises the following steps: introducing a sequence for coding BT toxin into halomonas, then culturing the halomonas in a culture medium and inducing BT toxin expression, and the concentration of inorganic salt in the culture medium is 5-10% (w/v). According to the application, the halomonas is taken as host bacteria for producing the BT toxins, stable and high-yield BT toxins are realized by adjusting the temperature of culture and induced expression, and the problem of drug resistance can be solved by simultaneously expressing multiple BT toxins. In the halomonas culture process, sylvite is used as an inorganic salt for culture to obtain a culture solution which can be directly sprayed to crops, K ions are supplemented to the crops while insects are killed, the fertilization effect is achieved, and the fertilizer cost is reduced; and in addition, the subsequent wastewater treat