Humanized design and expression verification of murine HA tag antibody

The invention discloses an anti-HA tag humanized antibody and expression verification thereof. The anti-HA tag humanized antibody comprises a heavy chain FR1, a heavy chain FR2, a heavy chain FR3, a heavy chain FR4 or a heavy chain of a variant thereof, and a light chain of a light chain FR1, a ligh...

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Bibliographische Detailangaben
Hauptverfasser:	CHEN DINGRUI, HUANG DELI, ZHU-WAN YINHUI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an anti-HA tag humanized antibody and expression verification thereof. The anti-HA tag humanized antibody comprises a heavy chain FR1, a heavy chain FR2, a heavy chain FR3, a heavy chain FR4 or a heavy chain of a variant thereof, and a light chain of a light chain FR1, a light chain FR2, a light chain FR3, a light chain FR4 or a variant thereof, the amino acid sequence of the heavy chain FR1 comprises SEQ ID NO: 1 or consists of SEQ ID NO: 1; the heavy chain FR2 amino acid sequence comprises SEQ ID NO: 2 or consists of SEQ ID NO: 2; the amino acid sequence of the heavy chain FR3 comprises SEQ ID NO: 3 or consists of SEQ ID NO: 3; the heavy chain FR4 amino acid sequence comprises SEQ ID NO: 4 or consists of SEQ ID NO: 4; the amino acid sequence of the light chain FR1 comprises SEQ ID NO: 5 or consists of SEQ ID NO: 5; according to the present invention, the human-derived HA tag antibody structure is more similar to the human antibody through the gene recombination and optimization durin