QPCR (quantitative polymerase chain reaction) micro-fluidic chip card and use method

The invention relates to a qPCR (quantitative polymerase chain reaction) micro-fluidic chip card and a use method. The qPCR micro-fluidic chip card comprises a micro-fluidic chip body, the sample pretreatment part and the reaction cavity are communicated with the mixing part; the first exhaust flow channel is used for communicating the reaction cavity with the outside so as to exhaust gas in the reaction cavity; the first breathable waterproof piece is positioned between the first exhaust flow channel and the reaction cavity; the micro-fluidic chip body comprises a plugging area, at least part of the first exhaust flow channel is located in the plugging area, at least part of the first exhaust flow channel located in the plugging area can be sealed in a heating and/or extrusion deformation mode, and the plugging area is heated and/or extruded, so that the first exhaust flow channel is sealed. The first exhaust flow channel in the blocking area is blocked, and during amplification, aerosol generated by heating