Oil-in-water adjuvant as well as preparation method and application thereof

The invention provides an oil-in-water adjuvant as well as a preparation method and application thereof, and belongs to the technical field of biological product animal vaccines. The provided oil-in-water adjuvant is prepared from the following raw materials in percentage by mass: 75wt%-83wt% of oil for injection, 11wt%-15wt% of refined span-80, 6wt%-9wt% of refined tween-80 and 0.1 wt%-1wt% of an immunopotentiator. The vaccine prepared from the oil-in-water adjuvant provided by the invention is low in viscosity, stable in quality and high in safety, can induce an organism to generate long-lasting and efficient immune response, and can be used as a safe and effective adjuvant for livestock vaccines and the like. 本发明提供一种水包油佐剂及其制备方法和应用，属于生物制品类动物疫苗技术领域。提供的水包油佐剂的原料按质量百分比计包括：75wt％-83wt％的注射用油、11wt％-15wt％的精制司本-80、6wt％-9wt％的精制吐温-80和0.1wt％-1wt％的免疫增强剂。本发明提供的水包油佐剂制备的疫苗黏度低、质量稳定、安全性高，且能够诱导机体产生持续期长且高效的免疫反应，可用作畜类动物疫苗等的安全有效的佐剂。