Quality preservation method for preparing multi-vitamin polypeptide co-loaded nano-liposome eye protection patch

The invention discloses a quality guarantee method for preparing a multivitamin polypeptide co-loaded nano-liposome eye protection patch, which is characterized by comprising the following steps: slowly injecting absolute ethyl alcohol solutions of vitamin A, vitamin E, soybean lecithin, cholesterol...

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1. Verfasser: ZHU YANGGEN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a quality guarantee method for preparing a multivitamin polypeptide co-loaded nano-liposome eye protection patch, which is characterized by comprising the following steps: slowly injecting absolute ethyl alcohol solutions of vitamin A, vitamin E, soybean lecithin, cholesterol and the like into a phosphate buffer solution which is heated to 45-60 DEG C and has a pH value of 6.4-7.8 to prepare liposome, and stirring until ethanol is completely removed; dissolving vitamin B12, vitamin B6, small-molecule active polypeptide and the like in a phosphate buffer solution with the pH value of 6.4-7.8, adding into the liposome solution, heating to 45-70 DEG C on a water bath, preserving heat and hatching for 25-60 minutes to prepare a co-loaded liposome, adding a liposome stabilizer, homogenizing at high pressure, and filtering by a 0.22 m filter membrane; adding an aqueous solution of extracts of vaccinium myrtillus, chrysanthemum and the like, which are pretreated by boiling, dissolving, preser