Method for detecting contents of various lipid components in ganoderma lucidum spore oil by quantitative analysis of multi-components by single marker

The invention belongs to the technical field of quality standards and detection of health-care foods, and discloses a method for detecting the contents of various lipid components in ganoderma lucidum spore oil by a quantitative analysis of multi-components by single marker. According to the method, a high performance liquid chromatography method is adopted, triolein is taken as an internal reference substance, correction factors of triolein and other lipid components are established, and the concentrations of various to-be-detected components in the lipid in the ganoderma lucidum spore oil are calculated by utilizing the correction factors. According to the high performance liquid chromatography method, acetonitrile-acetone-isooctane is adopted as a mobile phase, and HPLC-UV combination is high in specificity. 本发明属保健食品的质量标准及其检测技术领域，公开了一测多评法检测灵芝孢子油中多种脂质成分含量的检测方法。本发明采用高效液相色谱法，以甘油三油酸酯为内参物，建立甘油三油酸酯与其他脂质成分的校正因子，利用校正因子计算灵芝孢子油中所述脂质中的多种待测组分的浓度。本发明高效液相色谱法采用乙腈-丙酮-异辛烷作为流动相、HPLC-UV联用专属性强。