High performance liquid chromatography for separating and detecting magnesium isoglycyrrhizinate and related substances thereof and application thereof

The invention provides a high performance liquid chromatography method for separating and detecting magnesium isoglycyrrhizinate and related substances thereof and application thereof. Wherein the stationary phase of a chromatographic column is octadecyl silane bonded silica gel, the mobile phase is dipotassium phosphate solution-acetonitrile, and the related substance is any one or a combination of a related substance 1, a related substance 2, a related substance 3, a related substance 4, a related substance 5, a related substance 6, a related substance 7, a related substance 8 and a related substance 9. The method provided by the invention can realize efficient separation and detection of magnesium isoglycyrrhizinate and related substances thereof, and can effectively control drug quality. 本发明提供一种分离并检测异甘草酸镁及其有关物质的高效液相色谱法及其应用。其中色谱柱固定相为十八烷基硅烷键合硅胶，流动相为磷酸氢二钾溶液-乙腈，所述有关物质选自有关物质1、有关物质2、有关物质3、有关物质4、有关物质5、有关物质6、有关物质7、有关物质8和有关物质9中的任一种或其组合。本发明的方法能实现异甘草酸镁及其有关物质的高效分离与检测，能有效控制药品质量。