Method for high-throughput screening of exopolysaccharide high-yield strain based on micro-viscosity molecular rotor

The invention belongs to the technical field of food biology, and particularly relates to a method for high-throughput screening of an exopolysaccharide high-yield strain based on a micro-viscosity molecular rotor. According to the method, a mixed solution is obtained by mixing a microbial fermentat...

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Bibliographische Detailangaben
Hauptverfasser: WANG YING, WANG FAN, SHAN CHENGJUN, FAN LINLIN, ZHU YONGSHENG, WANG XINGNA, ZHANG YU, ZHANG HONGZHI, LIU XIAOLI, XIAYIDAN MAIMAITI, WU HAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of food biology, and particularly relates to a method for high-throughput screening of an exopolysaccharide high-yield strain based on a micro-viscosity molecular rotor. According to the method, a mixed solution is obtained by mixing a microbial fermentation solution and a molecular rotor, and the exopolysaccharide production capacity of the microorganism is judged by measuring the fluorescence intensity of the mixed solution. A molecular rotor exploration fluorescence technology is used for more efficiently researching polysaccharide gel mechanical characteristics of a microbial fermentation system, a molecular rotor is adopted as an embedded sensor of a polysaccharide system, and fluorescence data is combined with traditional rheological detection, so that a high-throughput method for rapidly evaluating the exopolysaccharide production capacity of microorganisms is established. Extraction and determination of polysaccharide in fermentation liquor are not needed,