Construction method and application of intracellular high-yield L-lysine engineering bacteria based on cellulosome

The invention provides a construction method and application of intracellular high-yield L-lysine engineering bacteria based on cellulosome, and belongs to the field of gene engineering and enzyme engineering.The construction method comprises the steps that butt joint protein DocA-S3 and conglutinin Coh are utilized for assembling two enzyme gene combinations, a plasmid expression vector is constructed, the plasmid expression vector is transformed into escherichia coli, and the engineering bacteria are obtained; the escherichia coli engineering bacteria are obtained; the two enzyme gene combinations comprise a combination of aspC and lysC, a combination of lysC and asd, a combination of asd and dapA, a combination of dapA and dapB, a combination of dapB and dapE, a combination of dapE and dapF or a combination of dapF and lysA; according to the invention, the fibrosome docking protein mutant DocA-S3 is utilized to realize effective assembly with fibrosome conglutinin in cells, and the engineering bacterium pr