Enhanced mycoplasma ovipneumoniae antigen protein TU-sheep pox virus recombinant multivalent vaccine and construction method thereof

The invention belongs to the field of immune engineering, and particularly discloses an enhanced type mycoplasma ovipneumoniae antigen protein TU-sheep pox virus recombinant multivalent vaccine and a construction method thereof.An antigen gene TU of mycoplasma ovipneumoniae is selected, and two non-specific immune genes HSP70 and I FN-gamma are added to enhance the immune effect of the antigen gene TU; the method comprises the following steps: respectively carrying out biological information analysis on goat pox virus fragments, selecting regions with strong antigenicity for gene synthesis, connecting the three gene fragments together, and respectively inserting the three gene fragments into unmarked goat pox virus expression vectors for expressing exogenous genes by using a homologous recombination method, a sheep pox virus vaccine strain recombinant multivalent vaccine expression vector for expressing three antigen genes of the mycoplasma ovipneumoniae is constructed, and then the recombinant sheep pox viru