DNA Marker, kit, method and application for rapidly detecting fen-flavor liquor fungi

The invention relates to the technical field of microbial molecular ecology detection, and particularly discloses a DNA Marker, a kit and a method for rapidly detecting fen-flavor white spirit fungi and application of the DNA Marker, and the nucleotide sequence of the DNA Marker is shown as SEQ ID N...

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Bibliographische Detailangaben
Hauptverfasser: HAN YING, ZHEN PAN, HOU YIKE, WANG JUNYAN, YANG XIUQING, SHI BINBIN, WANG XIAOYONG, NIE ZHIQIANG, GENG TIANPEI
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to the technical field of microbial molecular ecology detection, and particularly discloses a DNA Marker, a kit and a method for rapidly detecting fen-flavor white spirit fungi and application of the DNA Marker, and the nucleotide sequence of the DNA Marker is shown as SEQ ID NO.4-SEQ ID NO.20. The invention further discloses a method for rapidly detecting fen-flavor white spirit fungi. According to the DNA Marker provided by the invention, the bacterial genus status of sample DNA is accurately represented through a species specific sequence (ITS1 region) of the DNA Marker, each composition band of the Marker is a single sequence, represents a specific strain and is only limited to use in denaturing gradient gel electrophoresis, the result is reliable, the specificity is good, and the method is suitable for large-scale popularization and application. The method is high in sensitivity, safe and visual, and a plurality of samples can be analyzed at the same time and the difference of the s