Method for separating plant embryo cell nucleus

The invention belongs to the technical field of agricultural biology, and particularly relates to a method for separating plant embryo cell nucleuses. According to the method disclosed by the invention, plant embryos with high grease and high starch content are subjected to splitting decomposition and solid-liquid separation, so that plant cell nucleuses are fully released; the cell nucleus suspension is subjected to Percoll solution density gradient centrifugation, so that more complete cell nucleuses can be obtained through separation. Experiments prove that compared with a flow cytometry sorting method, after the pinus tabulaeformis embryos are separated by adopting the technical scheme disclosed by the invention, the integrity rate of cell nucleuses reaches up to 85.9% and is 7.7% higher than that of a control group. Therefore, according to the technical scheme provided by the invention, the plant embryo cell nucleus with high lipid content and high starch content can be obtained through efficient separat