Method and device for rapidly detecting patulin in fruit and vegetable juices and beverages thereof

The invention discloses a rapid detection method and a detection device for patulin in fruit and vegetable juices and beverages thereof, and relates to a patulin derivative, antigen, antibody and colloidal gold detection device and preparation thereof, and a rapid detection method for detecting patu...

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Bibliographische Detailangaben
Hauptverfasser: MO QIUHUA, LIU YUNWANG, LIANG QIUYI, WANG SHUISHU, WANG BINGZHI, LI XIQING, KO YOUNG-GUN, XIAO ZHAOLI, YANG XINGXING, WANG WEIWEI, DENG QIFU, QIN DONGMEI, FU HUI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a rapid detection method and a detection device for patulin in fruit and vegetable juices and beverages thereof, and relates to a patulin derivative, antigen, antibody and colloidal gold detection device and preparation thereof, and a rapid detection method for detecting patulin in fruit and vegetable juices and beverages thereof. The invention provides an artificial antigen prepared by coupling a patulin derivative with a carrier protein, and an antibody which is generated by a body of an immunized experimental animal and specifically aims at the patulin derivative, the linear detection range of the antibody to the patulin derivative is 0.06-2.37 ng/mL, and the sensitivity is 0.383 ng/mL. The method is used for exclusive detection of patulin in fruit and vegetable juices and beverages thereof, has the advantages of being good in specificity, high in sensitivity, easy and convenient to operate and the like, can better meet the requirements of self-inspection of production enterprises a