Preparation method and application of biological hybrid free radical generator based on streptococcus pneumoniae

The invention relates to a preparation method and application of a biological heterozygous free radical generator based on streptococcus pneumoniae, the biological heterozygous free radical generator can continuously generate high-activity OH to efficiently kill tumors without being limited by tumor hypoxia, and the technical scheme for solving the problem is that the biological heterozygous free radical generator comprises the following steps: 1) preparing azide modified streptococcus pneumoniae S.pn-N3; (2) preparing an alkynyl modified Fe3O4 nano particle (Fe3O4-DBCO); (3) preparing a biological hybrid free radical generator Fe3O4 (at) S.pn based on streptococcus pneumoniae; the method is convenient to operate, stable and reliable, the prepared tumor biological hybrid free radical generator has the advantages of being high in selectivity, continuous, efficient and the like, the limitation of a tumor hypoxic microenvironment can be broken through in the aspect of tumor treatment, high-activity OH can be sel