Enzyme activity detection method of carbonyl reductase
The invention relates to an enzyme activity detection method of carbonyl reductase, and belongs to the technical field of enzyme activity analysis. In order to solve the problem of complex enzyme activity detection in the prior art, the invention provides a carbonyl reductase enzyme activity detecti...
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| creator | ZHANG LIWEI SU JIAN JIANG ZHIYONG YANG JINRUN CHEN TIAN |
| description | The invention relates to an enzyme activity detection method of carbonyl reductase, and belongs to the technical field of enzyme activity analysis. In order to solve the problem of complex enzyme activity detection in the prior art, the invention provides a carbonyl reductase enzyme activity detection method, which is characterized by comprising the following steps: adding coenzyme and carbonyl reductase into a reaction system of a substrate compound shown as a formula I, isopropanol and a triethanolamine buffer solution; reacting under the condition that the temperature is controlled to be 30-38 DEG C to prepare a product compound as shown in a formula II; the protein sequence of the carbonyl reductase is as shown in SEQ ID NO. 1; in the reaction process, sampling after preset reaction time to analyze the content of the substrate compound shown as the formula I in the reaction liquid, and calculating the enzyme activity according to the conversion rate of the substrate compound shown as the formula I to obta |
| format | Patent |
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In order to solve the problem of complex enzyme activity detection in the prior art, the invention provides a carbonyl reductase enzyme activity detection method, which is characterized by comprising the following steps: adding coenzyme and carbonyl reductase into a reaction system of a substrate compound shown as a formula I, isopropanol and a triethanolamine buffer solution; reacting under the condition that the temperature is controlled to be 30-38 DEG C to prepare a product compound as shown in a formula II; the protein sequence of the carbonyl reductase is as shown in SEQ ID NO. 1; in the reaction process, sampling after preset reaction time to analyze the content of the substrate compound shown as the formula I in the reaction liquid, and calculating the enzyme activity according to the conversion rate of the substrate compound shown as the formula I to obta</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240223&DB=EPODOC&CC=CN&NR=117587096A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240223&DB=EPODOC&CC=CN&NR=117587096A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHANG LIWEI</creatorcontrib><creatorcontrib>SU JIAN</creatorcontrib><creatorcontrib>JIANG ZHIYONG</creatorcontrib><creatorcontrib>YANG JINRUN</creatorcontrib><creatorcontrib>CHEN TIAN</creatorcontrib><title>Enzyme activity detection method of carbonyl reductase</title><description>The invention relates to an enzyme activity detection method of carbonyl reductase, and belongs to the technical field of enzyme activity analysis. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Enzyme activity detection method of carbonyl reductase |
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