Enzyme activity detection method of carbonyl reductase

The invention relates to an enzyme activity detection method of carbonyl reductase, and belongs to the technical field of enzyme activity analysis. In order to solve the problem of complex enzyme activity detection in the prior art, the invention provides a carbonyl reductase enzyme activity detection method, which is characterized by comprising the following steps: adding coenzyme and carbonyl reductase into a reaction system of a substrate compound shown as a formula I, isopropanol and a triethanolamine buffer solution; reacting under the condition that the temperature is controlled to be 30-38 DEG C to prepare a product compound as shown in a formula II; the protein sequence of the carbonyl reductase is as shown in SEQ ID NO. 1; in the reaction process, sampling after preset reaction time to analyze the content of the substrate compound shown as the formula I in the reaction liquid, and calculating the enzyme activity according to the conversion rate of the substrate compound shown as the formula I to obta