Recombinant hexokinase as well as preparation method and application thereof

The invention discloses recombinant hexokinase as well as a preparation method and application thereof. The invention provides a polynucleotide sequence which is optimized by synonymous codon preference and is used for coding hexokinase. The sequence has high expression efficiency in escherichia coli; the invention also provides a carrier adapted to the polynucleotide sequence, high-activity hexokinase is prepared through synergistic cooperation of the carrier and the polynucleotide sequence, and the specific activity is as high as 111.8 U/mg; in addition, the invention further provides a method for preparing the recombinant hexokinase, the method not only improves the yield of the hexokinase, but also enables the obtained product to be extremely high in soluble protein content and easy to purify, the post-treatment step is simplified, and the production cost is saved. 本申请公开了一种重组己糖激酶及其制备方法和应用。本申请中提供了经同义密码子偏好性优化的编码己糖激酶的多核苷酸序列，该序列在大肠杆菌中表达效率高；还提供了适配上述多核苷酸序列的载体，通过载体和多核苷酸序列的协同配合，制备获得高活性己糖激酶，比活力高达111.8U/mg；此外还提供了制备