Recombinant uricase as well as preparation method and application thereof

The invention discloses recombinant uricase as well as a preparation method and application thereof. The invention provides a polynucleotide sequence which is optimized by synonymous codon preference and is used for coding uricase, and the sequence has high expression efficiency in escherichia coli; the invention also provides a carrier adapted to the polynucleotide sequence, the high-activity uricase is prepared through the synergistic cooperation of the carrier and the polynucleotide sequence, and the specific activity is as high as 32.2 U/mg; in addition, the invention further provides a method for preparing the recombinant uricase, the yield of the uricase is increased, the content of soluble protein in the obtained product is extremely high, the product is easy to purify, post-treatment steps are simplified, and the production cost is saved. 本申请公开了一种重组尿酸酶及其制备方法和应用。本申请中提供了经同义密码子偏好性优化的编码尿酸酶的多核苷酸序列，该序列在大肠杆菌中表达效率高；还提供了适配上述多核苷酸序列的载体，通过载体和多核苷酸序列的协同配合，制备获得高活性尿酸酶，比活力高达32.2U/mg；此外还提供了制备重组尿酸酶的方法，该方法不仅提高了尿酸酶的产量，而且所