Novel recombinant factor C as well as preparation method and application thereof

The invention belongs to the field of endotoxin detection, and particularly discloses a novel recombinant factor C as well as a preparation method and application thereof. According to the invention, an existing horseshoe crab blood coagulation C factor is modified, the original sequence (functional domain) of a light chain region is kept unchanged, and a Sushi1 region and a Sushi2 region in a heavy chain are replaced by a Sushi4 region in the light chain. On the premise that the overall structure and functional domain of Factor C are not changed, the free energy of the protein is reduced, the protein is more stable, the substrate binding capacity is improved, a signal sequence is added to the N end of the protein sequence, so that the target protein is expressed extracellularly, follow-up detection and purification are facilitated, and finally the modified protein rFC-MBJ is designed. The expression is easier, the yield is high and the effect is good. 本发明属于内毒素检测领域，具体公开了一种新型重组C因子及其制备方法和应用。本发明对现有鲎凝血C因子进行改造，维持轻