Chicken embryo fibroblast suspension culture method

The invention discloses a chick embryo fibroblast suspension culture method, which comprises the following steps: treating a 10-day-old chick embryo into chick embryo digestive juice, deenzyming the chick embryo digestive juice, adding a culture medium with 5% fetal calf serum, placing the chick embryo digestive juice and the culture medium into a culture bottle, placing the culture bottle into a magnetic stirrer for stirring, standing and precipitating, and culturing the chick embryo fibroblast in the culture bottle. The method comprises the following steps of: adding a culture flask into a culture medium, adding trypsin into the culture flask, digesting for multiple times, counting cells, storing at 2-8 DEG C, and diluting the cells until the cell density is (1.0-2.0) * 10 < 6 > cells/ml before the cells are transferred into a bioreactor; and carrying out suspension culture in a bioreactor. According to the suspension culture method, the number of effective cells in unit volume can be obviously increased, t