Targeting nano-molecular probe as well as preparation method and application thereof

The invention relates to the technical field of biological medicines, in particular to a targeting nano molecular probe as well as a preparation method and application thereof. Receptor plasmids corresponding to immune checkpoints for stably expressing tumor surface negative regulation molecules are synthesized, and the receptor plasmids, cell membrane nano-vesicles which enhance T cell active ligands and are labeled by fluorescence, and melanin nano-spheres which simultaneously entrap therapeutic drugs are used for preparing the targeted nano-molecular probe. The targeting nano-molecular probe provided by the invention can perform long circulation in vivo, so that the dosage and the administration frequency of chemotherapeutic drugs are reduced. The medicine resistance of a tumor part to a treatment medicine is improved, tumor DNA damage is targeted, and infiltration and activation of immune cells of the tumor part are obviously enhanced. 本发明涉及生物医药技术领域，具体涉及一种具有靶向性的纳米分子探针及其制备方法与应用。本发明合成稳定表达肿瘤表面负调控分子的免疫检查点对应受体