Target gene for detecting influenza A virus, primer probe combination, kit and application

The invention provides a target gene for detecting influenza A virus, a primer probe combination, a kit and application. The sequence of a marker has a sequence shown in SEQ ID NO.1 or a specific fragment. The dual IAV detection method provided by the invention is short in detection time, simple in instrument and equipment, low in experiment cost and free of temperature change conditions, and the method contains an internal reference primer pair and an internal reference probe, so that false negative and invalid results are eliminated, the method has good specificity and sensitivity, the analysis sensitivity reaches 1 copy/mu L, and the method is suitable for detection of a large number of samples. 本发明提供了一种检测甲型流感病毒的靶基因、引物探针组合以及试剂盒和应用，标志物的序列具有SEQ ID NO.1所示序列或特异性片段。本发明所提供的双重IAV检测方法，检测用时短、仪器设备简单、实验成本低、无需变温条件，而且本发明方法中含有了内参引物对和内参探针排除了假阴性和无效结果，具有良好的特异性和灵敏性，分析灵敏度达到1拷贝/μL，适用于大量样本检测。