Fish pathogenic microorganism aeromonas hydrophila 16SrRNA gene detection kit based on hybrid chain reaction (HCR) and application thereof

The invention relates to a fish pathogenic microorganism aeromonas hydrophila 16SrRNA gene detection kit based on a hybrid chain reaction (HCR) and application of the fish pathogenic microorganism aeromonas hydrophila 16SrRNA gene detection kit. The fish pathogenic microorganism aeromonas hydrophila 16SrRNA gene detection kit comprises a determined aeromonas hydrophila specific DNA sequence 16S ribosomal RNA, a pair of hairpin primers 16SrRNA-H1 and 16SrRNA-H2, a fluorophore, a quenching group, a DNA storage solution and a CaCl2 solution. When in use, a water body sample or bacterial liquid prepared by separating visceral organs and tissues of sick fishes is taken, and the H1 primer and the H2 primer are added; standing on ice, and performing constant-temperature heat shock at 42 DEG C; after heat shock, rapidly placing on ice for cooling, adding DNA storage liquid, placing at room temperature, and detecting by using a fluorescence microscope. And judging whether the sample to be detected contains aeromonas h