Serum-free culture medium suitable for CIK cell culture and culture method thereof
The invention discloses a serum-free culture medium suitable for CIK cell culture and a culture method thereof, and relates to the technical field of immune cells, the serum-free culture medium suitable for CIK cell culture comprises an ALyS505N-0/TAKARA GT-T551 serum-free culture medium with a volu...
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| creator | LIU JUNXIANG KE YINGJIA ZHONG GUIQIANG XING LAN JIANG JINXIAN WANG QINGFANG LIU BINGHONG |
| description | The invention discloses a serum-free culture medium suitable for CIK cell culture and a culture method thereof, and relates to the technical field of immune cells, the serum-free culture medium suitable for CIK cell culture comprises an ALyS505N-0/TAKARA GT-T551 serum-free culture medium with a volume fraction of 94.5%, and further comprises the following components according to a concentration, the culture medium comprises the following components in percentage by volume: 1% of MEM vitamin solution, 5 mM of L-glutamine, 1% of NEAA, 1.5 g/L of NaHCO3, 0.1% of GP type defoaming agent, 2% of sterile 50% glucose mother liquor, 0.5% of phenol red solution, 0.1% of water, 250 iu/mL of IL-1, 2300 iu/mL of IL-2, 35 g/L of OKT3 and 4 mg/L of IFN-g. The provided serum-free culture medium suitable for CIK cell culture does not contain fetal calf serum, so that the probability of cell infection diseases and pollution is reduced; meanwhile, the serum-free culture medium provided by the invention also plays a role in prom |
| format | Patent |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Serum-free culture medium suitable for CIK cell culture and culture method thereof |
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