Serum-free culture medium suitable for CIK cell culture and culture method thereof

The invention discloses a serum-free culture medium suitable for CIK cell culture and a culture method thereof, and relates to the technical field of immune cells, the serum-free culture medium suitable for CIK cell culture comprises an ALyS505N-0/TAKARA GT-T551 serum-free culture medium with a volume fraction of 94.5%, and further comprises the following components according to a concentration, the culture medium comprises the following components in percentage by volume: 1% of MEM vitamin solution, 5 mM of L-glutamine, 1% of NEAA, 1.5 g/L of NaHCO3, 0.1% of GP type defoaming agent, 2% of sterile 50% glucose mother liquor, 0.5% of phenol red solution, 0.1% of water, 250 iu/mL of IL-1, 2300 iu/mL of IL-2, 35 g/L of OKT3 and 4 mg/L of IFN-g. The provided serum-free culture medium suitable for CIK cell culture does not contain fetal calf serum, so that the probability of cell infection diseases and pollution is reduced; meanwhile, the serum-free culture medium provided by the invention also plays a role in prom