Multiplex fluorescent quantitative PCR (Polymerase Chain Reaction) primer, probe and detection method for identifying African swine fever virus

The invention relates to the technical field of African swine fever virus detection, and discloses multiple fluorescent quantitative PCR primers, probes and a detection method for identifying African swine fever viruses, the fluorescent quantitative PCR primers comprise an ASFV P72 gene specific pri...

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Bibliographische Detailangaben
Hauptverfasser:	CHU YUANYUAN, HUANG YING, TAO CHUNHAO, ZHAI WENZHU, JIA HONG, HE YUHENG, PANG ZHONGBAO, YUAN WEIFENG, ZHU HONGFEI, WANG ZHEN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the technical field of African swine fever virus detection, and discloses multiple fluorescent quantitative PCR primers, probes and a detection method for identifying African swine fever viruses, the fluorescent quantitative PCR primers comprise an ASFV P72 gene specific primer, an ASFV CD2v gene specific primer and an ASFV MGF 360-12L gene specific primer, and the ASFV P72 gene specific primer, the ASFV CD2v gene specific primer, the ASFV MGF 360-12L gene specific primer and the ASFV MGF 360-12L gene specific primer are used for identifying the African swine fever viruses. The ASFV P72 gene specific primer comprises a forward primer P72-L and a reverse primer P72-R of a nucleotide sequence, the ASFV CD2v gene specific primer comprises a forward primer CD2v-L and a reverse primer CD2v-R of a nucleotide sequence, and the ASFV MGF 360-12L gene specific primer comprises a forward primer MGF 360-12L-L and a reverse primer MGF 360-12L-R of a nucleotide sequence. According to the method, th