Related amplification primer for detecting common pathogenic bacteria in feed and raw materials, kit and application thereof

The invention discloses related amplification primers and a kit for detecting common pathogenic bacteria in feed and raw materials and application of the related amplification primers and the kit. The invention specifically discloses a multiplex PCR primer composition for identification or auxiliary identification of common pathogenic bacteria, and the primer composition comprises a primer pair A-K, the method comprises the following steps: respectively carrying out specific amplification on salmonella, staphylococcus aureus, escherichia coli O157: H7, shigella, campylobacter jejuni, listeria monocytogenes, clostridium perfringens, vibrio parahaemolyticus, a bacillus cereus bceT gene, an entFM gene and a wax gyrB gene; corresponding probes A-K are matched to realize detection of common pathogenic bacteria in a sample by a gene membrane chip method through a reverse dot blot hybridization technology. The method has the advantages of simplicity, high detection speed, high specificity, low detection limit, high