Behavior trajectory tracking kit and tracking method for releasing acipenser gurgeons at upstream of Yangtze River based on probe method

The invention discloses a probe method-based behavior trajectory tracking kit and tracking method for acipenser gurgeon released at the upstream of the Yangtze River, and the method comprises the following steps: analyzing mitochondrial DNA sequences of acipenser gurgeon and common fishes in the Yangtze River basin, designing and screening to obtain a specific primer pair ND5-F4/ND5-R4 capable of specifically amplifying the mitochondrial DNA of the acipenser gurgeon and a Taqman fluorescent probe ND5 Probe4; the kit is composed of specific primers, Taqman fluorescent probes and ddPCR premixed liquid, ddPCR detection is conducted on collected environment DNA samples before and after releasing, the behavior track of released Yangtze river sturgeons can be tracked and monitored, non-contact type Yangtze river sturgeons' behavior track tracking is achieved, damage to fish bodies in the tracking process is avoided, the tracking cost is reduced, and the tracking efficiency is improved. And the overall tracking accu