Method for increasing 2n pollen occurrence rate of tabebuia chrysantha

The invention discloses a method for increasing the 2n pollen occurrence rate of tabebuia chrysantha. The induction method and the identification method for the Tabebuia chrysantha 2n pollen are established for the first time, the research shows that the occurrence rate of the Tabebuia chrysantha 2n pollen can be increased by adopting colchicine treatment, and the occurrence rate of the 2n pollen can be obviously increased by 0.2% colchicine treatment and reaches 1.36%. According to the method, colchicine is adopted to infiltrate tabebuia chrysantha buds in the pollen mother cell stage, the 2n gamete occurrence rate is increased through a non-invasive treatment method, the possibility is provided for cultivating a new variety of sexual polyploidy tabebuia chrysantha through a 2n gamete approach, and the method has important significance for further promoting breeding of the sexual polyploidy of tabebuia chrysantha. 本发明公开了一种提高黄花风铃木2n花粉发生率的方法。本发明首次建立了黄花风铃木2n花粉诱导方法与鉴定方法，研究显示采用秋水仙素处理能提高黄花风铃木2n花粉发生率，其中0.2％秋水仙素处理能显