Culture kit for NK cells targeting lung and highly expressing NKG2D and application of culture kit

The invention discloses a culture kit of NK cells targeting lungs and highly expressing NKG2D and application of the culture kit. The kit comprises an activation culture medium; the activation culture medium is a basic culture medium containing at least one of interleukin, stem cell factors, interferon, a BMP signal channel activator, vitamins, glucocorticoid, a tyrosine kinase inhibitor, an interleukin receptor agonist, a TLR agonist and autoplasma. The kit provided by the invention can be used for culturing a group of NK cells which target lung and highly express the NKG2D receptor, and can be used for solving the problems of poor targeting killing activity caused by weak NK cell tissue targeting and low NKG2D receptor expression in NK cell in-vitro amplification in the prior art. 本发明公开了一种靶向肺部且高表达NKG2D的NK细胞的培养试剂盒及其应用，所述试剂盒包括活化培养基；所述活化培养基为包含以下至少一种的基础培养基：白细胞介素、干细胞因子、干扰素、BMP信号通路激活剂、维生素、糖皮质激素、酪氨酸激酶抑制剂、白细胞介素受体激动剂、TLR激动剂、自体血浆。本发明提供的试剂盒能够培养出一群靶向肺部且高表达NKG2D受体的NK细胞，可以用于解决现有技术中NK细胞体外扩增中存在的NK细胞组织靶向性弱、NKG2D受体表达低导致靶向性杀伤