Particle-based immunoassays using pegylated analyte-specific binding agents

Herein is reported a method for measuring an analyte in a microparticle-based analyte-specific binding assay wherein the microparticle is coated with a first partner of a binding pair, the method comprises mixing the coated microparticles, an analyte-specific binding agent conjugated to a second partner of a binding pair, and a sample suspected of comprising or comprising an analyte wherein the second partner of a binding pair is bound to the analyte-specific binding agent via a linker comprising 12 to 30 ethylene glycol units (PEG 12 to 30), the coated microparticle is coated with a binding pair, whereby the analyte is bound to the coated microparticle via the conjugated analyte-specific binding agent, the microparticle comprising the analyte bound via the binding pair and the analyte-specific binding agent is separated from the mixture, and the analyte bound to the microparticle is measured. 本文报道了用于在基于微粒的分析物特异性结合测定中测量分析物的方法，其中所述微粒涂覆有结合对的第一配偶体，所述方法包括混合所述涂覆的微粒、缀合至结合对的第二配偶体的分析物特异性结合剂和怀疑包含分析物或包含分析物的样品，其中结合对的所述第