Quenching device for fluorescence quenching, differential fluorescence quenching method and application

The invention provides a quenching device for fluorescence quenching. The quenching device comprises a light source; the optical filter is arranged below the light emitted by the light source; the transparent object containing device is arranged below the optical filter; and the light reflecting device is arranged below the transparent object containing device. According to the fluorescence quenching device provided by the invention, rapid and accurate fluorescence quenching can be realized, the quenching of inherent fluorescence molecules of a sample is irreversible, and the quenching effect cannot be weakened due to any washing treatment in the later dyeing process. A background fluorescence quenching system does not influence the brightness of specific fluorescent molecules marked in the later period at all, on the contrary, due to complete removal of background fluorescence, the signal-to-noise ratio and specificity of specific fluorescent marks can be further enhanced, and the better signal-to-noise rati