Chondrocyte culture method for promoting chondrocyte proliferation and maintaining chondrocyte phenotype and application
The invention relates to a chondrocyte culture method capable of promoting chondrocyte proliferation and maintaining chondrocyte phenotypes. The method is realized by introducing modified citrus pectin (MCP) into a cartilage cell culture system, MCP is independently added into a cartilage cell cultu...
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| creator | ZHANG YIYUN CHEN YAZHEN DUAN RUIPING DU BO LIU LINGRONG LI XUEMIN HE JIAYUE SU DANNING |
| description | The invention relates to a chondrocyte culture method capable of promoting chondrocyte proliferation and maintaining chondrocyte phenotypes. The method is realized by introducing modified citrus pectin (MCP) into a cartilage cell culture system, MCP is independently added into a cartilage cell culture solution, and the cartilage cells can also be planted in a biological material or a scaffold material of the composite MCP, so that the effect of the MCP on the cartilage cells is effectively utilized, and the cartilage cells can be cultured. Proliferation of cartilage cells is promoted, the phenotype of the cartilage cells is stably maintained, and the method can be used for construction of tissue engineered cartilage based on MCP and regeneration and repair of cartilage tissue defects.
本发明涉及一种具有促进软骨细胞增殖并维持软骨细胞表型的软骨细胞培养方法。该方法是通过在软骨细胞培养体系中引入改性柑橘果胶（MCP）实现，通过单独添加MCP至软骨细胞培养液中，也可以通过将软骨细胞种植于复合MCP的生物材料或支架材料，从而有效利用MCP对软骨细胞的作用，促进软骨细胞的增殖并稳定维持软骨细胞表型，该方法可用于基于MCP的组织工程化软骨构建和软骨组织缺损的再生修复。 |
| format | Patent |
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本发明涉及一种具有促进软骨细胞增殖并维持软骨细胞表型的软骨细胞培养方法。该方法是通过在软骨细胞培养体系中引入改性柑橘果胶（MCP）实现，通过单独添加MCP至软骨细胞培养液中，也可以通过将软骨细胞种植于复合MCP的生物材料或支架材料，从而有效利用MCP对软骨细胞的作用，促进软骨细胞的增殖并稳定维持软骨细胞表型，该方法可用于基于MCP的组织工程化软骨构建和软骨组织缺损的再生修复。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240202&DB=EPODOC&CC=CN&NR=117487744A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240202&DB=EPODOC&CC=CN&NR=117487744A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHANG YIYUN</creatorcontrib><creatorcontrib>CHEN YAZHEN</creatorcontrib><creatorcontrib>DUAN RUIPING</creatorcontrib><creatorcontrib>DU BO</creatorcontrib><creatorcontrib>LIU LINGRONG</creatorcontrib><creatorcontrib>LI XUEMIN</creatorcontrib><creatorcontrib>HE JIAYUE</creatorcontrib><creatorcontrib>SU DANNING</creatorcontrib><title>Chondrocyte culture method for promoting chondrocyte proliferation and maintaining chondrocyte phenotype and application</title><description>The invention relates to a chondrocyte culture method capable of promoting chondrocyte proliferation and maintaining chondrocyte phenotypes. The method is realized by introducing modified citrus pectin (MCP) into a cartilage cell culture system, MCP is independently added into a cartilage cell culture solution, and the cartilage cells can also be planted in a biological material or a scaffold material of the composite MCP, so that the effect of the MCP on the cartilage cells is effectively utilized, and the cartilage cells can be cultured. Proliferation of cartilage cells is promoted, the phenotype of the cartilage cells is stably maintained, and the method can be used for construction of tissue engineered cartilage based on MCP and regeneration and repair of cartilage tissue defects.
本发明涉及一种具有促进软骨细胞增殖并维持软骨细胞表型的软骨细胞培养方法。该方法是通过在软骨细胞培养体系中引入改性柑橘果胶（MCP）实现，通过单独添加MCP至软骨细胞培养液中，也可以通过将软骨细胞种植于复合MCP的生物材料或支架材料，从而有效利用MCP对软骨细胞的作用，促进软骨细胞的增殖并稳定维持软骨细胞表型，该方法可用于基于MCP的组织工程化软骨构建和软骨组织缺损的再生修复。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>DISINFECTION, STERILISATION, OR DEODORISATION OF AIR</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyzELwjAQBeAsDqL-h_MHOBQDcZWiODm5l5BcTCC9C-kV7L-3FAfBxeHx4PG9tXq1kclXdpMguDHLWBF6lMgeAlcolXuWRE9wX3BecwpYrSQmsOSht4lkzo-MSCxTwUXZUnJyy2urVsHmAXef3qj99fJobwcs3OFQrENC6dp70xh9Mkbr8_Ef8wbYkEhJ</recordid><startdate>20240202</startdate><enddate>20240202</enddate><creator>ZHANG YIYUN</creator><creator>CHEN YAZHEN</creator><creator>DUAN RUIPING</creator><creator>DU BO</creator><creator>LIU LINGRONG</creator><creator>LI XUEMIN</creator><creator>HE JIAYUE</creator><creator>SU DANNING</creator><scope>EVB</scope></search><sort><creationdate>20240202</creationdate><title>Chondrocyte culture method for promoting chondrocyte proliferation and maintaining chondrocyte phenotype and application</title><author>ZHANG YIYUN ; CHEN YAZHEN ; DUAN RUIPING ; DU BO ; LIU LINGRONG ; LI XUEMIN ; HE JIAYUE ; SU DANNING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117487744A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>DISINFECTION, STERILISATION, OR DEODORISATION OF AIR</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHANG YIYUN</creatorcontrib><creatorcontrib>CHEN YAZHEN</creatorcontrib><creatorcontrib>DUAN RUIPING</creatorcontrib><creatorcontrib>DU BO</creatorcontrib><creatorcontrib>LIU LINGRONG</creatorcontrib><creatorcontrib>LI XUEMIN</creatorcontrib><creatorcontrib>HE JIAYUE</creatorcontrib><creatorcontrib>SU DANNING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHANG YIYUN</au><au>CHEN YAZHEN</au><au>DUAN RUIPING</au><au>DU BO</au><au>LIU LINGRONG</au><au>LI XUEMIN</au><au>HE JIAYUE</au><au>SU DANNING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Chondrocyte culture method for promoting chondrocyte proliferation and maintaining chondrocyte phenotype and application</title><date>2024-02-02</date><risdate>2024</risdate><abstract>The invention relates to a chondrocyte culture method capable of promoting chondrocyte proliferation and maintaining chondrocyte phenotypes. The method is realized by introducing modified citrus pectin (MCP) into a cartilage cell culture system, MCP is independently added into a cartilage cell culture solution, and the cartilage cells can also be planted in a biological material or a scaffold material of the composite MCP, so that the effect of the MCP on the cartilage cells is effectively utilized, and the cartilage cells can be cultured. Proliferation of cartilage cells is promoted, the phenotype of the cartilage cells is stably maintained, and the method can be used for construction of tissue engineered cartilage based on MCP and regeneration and repair of cartilage tissue defects.
本发明涉及一种具有促进软骨细胞增殖并维持软骨细胞表型的软骨细胞培养方法。该方法是通过在软骨细胞培养体系中引入改性柑橘果胶（MCP）实现，通过单独添加MCP至软骨细胞培养液中，也可以通过将软骨细胞种植于复合MCP的生物材料或支架材料，从而有效利用MCP对软骨细胞的作用，促进软骨细胞的增殖并稳定维持软骨细胞表型，该方法可用于基于MCP的组织工程化软骨构建和软骨组织缺损的再生修复。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DISINFECTION, STERILISATION, OR DEODORISATION OF AIR ENZYMOLOGY HUMAN NECESSITIES HYGIENE MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES MEDICAL OR VETERINARY SCIENCE METALLURGY METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Chondrocyte culture method for promoting chondrocyte proliferation and maintaining chondrocyte phenotype and application |
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